{Author}: Chen Ming-Hong；Zhang Wen-Long；Chen Li；Lin Ru；Xie Yang；Fang Dong-Sheng；Lian Yun-Yang；Jiang Hong*

{Journal}: Natural product research

{Year}: 2019

{Volume}: 33

{Issue}: 20

{Pages}: 2897-2903

{Keywords}: Alkaloid；Antimicrobial activity；Marine sponge

{Abstract}: Chemical investigation of a marine-derived actinomycete strain Verrucosispora sp. FIM06025 isolated from a marine sponge sample collected from the East China Sea, resulted in the discovery of two new alkaloids, (2-(hydroxymethyl)-3-(2-(hydroxymethyl)-3-methylaziridin-1-yl) (2-hydroxyphenyl) methanone(1) and 2-(1-hydroxyethyl)-3,4-dihydrobenzo[f ] [1,4]oxazepin-5( 2H )-one ( 2 ). The structures of compounds 1 and 2 were determined by the detailed analysis of 1D, 2D NMR and HR-TOF-MS data, along with literature data analysis. The bioefficacy investigations revealed that compound 1 exhibited a broad spectrum of antimicrobial activity with MIC (minimum inhibitory concentration) values ranging from 3.4 to 200 μg·mL-1 against H. pylori, P. aeroginosa, A. baumanniiin, E. coli and K. pneumonia, S. aureus, C. albicans and E. faecium , however, compound 2 , up to 200 μg/mL, displayed no antibacterial activity against these bacteria.