{Author}: Yong ZHUANG；Kai HUANG；

{Journal}: Agricultural Biotechnology

{Year}: 2017

{Volume}: 6

{Issue}: 04

{Pages}: 67-68+72

{Keywords}: Iminodiacetic acid；N-(phosphonomethyl) iminodiacetic acid；High Performance Liquid Chromatography；Precolumn derivatization

{Abstract}: The derivatives of the reaction liquid for synthesizing N-(phosphonomethyl) iminodiacetic acid (PMIDA) were synthesized with 9-fluorenyl methyl chloroformate (FMOC-Cl) as the derivatization reagent. The separation of the derivatives was performed on a Gemini C(18) column at room temperature by isocratic elution. The mobile phase was composed of 60% 0.05 M NaH2PO4 (pH =3.5) and 40% CH3CN at a flow rate of 1 ml/min. The injection volume was 10 μl and the detection wavelength was 265 nm. The linear ranges of the derivatives of iminodiacetonitrile iminodiacetic acid (IDA) and ammonia were respectively 0.02-1.2 0.02-1.2 and0.1-2.0 mg/ml with correlation coefficients not smaller than 0.999 8. The detection limits for the derivatives were respectively 0.000 4 0.000 4 and 0.003 mg/ml.The average recoveries of 3 derivatives were respectively 99.96% 99.86% and 99.89% respectively with relative standard deviation values not smaller than 1.6%.The method developed in the present paper could be used for monitoring the reaction system for preparation of PMIDA by IDA.