{Author}:罗 婷 林永勉 郑孝贤 郑军荣 程元荣

{Year}:2013

{Title}:子囊霉素产生菌营养生物合成和化学合成限定培养基的建立

{Journal}:中国抗生素杂志

{Volume}:38

{Issue}:1

{Pages}: 26-31

{ISBN/ISSN}: 1001-8689

{Keywords}:子囊霉素 FK506 雷帕霉素 化学合成限定培养基 生物合成

{Abstract}:目的建立新型免疫抑制剂子囊霉素产生菌吸水链霉菌FAC0329的化学合成限定培养基,为进一步研究子囊霉素产生菌的营养生物合成奠定基础。方法分别试验不同浓度的20种碳源,19种氨基酸和7种无机盐对子囊霉素产生菌FAC0329生长和子囊霉素生物合成的影响,应用建立的子囊霉素合成培养基研究了子囊霉素、FK506和雷帕霉素三个结构类似物的生物合成,表明该培养基的专一性。结果与结论研究了子囊霉素的营养生物合成,首次建立了适合子囊霉素产生菌的专一化学合成限定培养基,为进一步研究子囊霉素的生物合成,提高发酵生物效价提供必要条件。

Objective To establish a chemical defined medium for ascomycin,a new containing nitrogen immunosuppressant,produced by Streptomyces hygroscopicus var.ascomyceticus FAC0329.Methods Effects of various concentrations of 20 carbon sources,19 amino acids and 7 inorganic salts on the producing strain were tested.Compared with FK506 and rapamycin,the established chemical defined medium was special for ascomycin producing strain.Results and conclusion Established a chemical defined medium of ascomycin t...