{Author}:吴云丹 聂毅磊 陈名洪 林 如 谢 阳 方东升 彭 飞 连云阳 江 红

{Year}:2014

{Title}:疣孢菌FIM090395细胞毒活性次级代谢产物的研究

{Journal}:《中国抗生素杂志》

{Volume}:39

{Issue}:2

{Pages}: 102-105

{ISBN/ISSN}:1001-8689

{Keywords}:疣孢菌 肿瘤细胞毒活性 次级代谢产物 Proximicins

{Abstract}:目的研究分离自海洋沉积物的疣孢菌FIM090395产生的次级代谢产物。方法采用HP-20大孔吸附树脂、正相硅胶、Sephadex LH-20葡聚糖凝胶和反相C18柱层析等方法,对菌株FIM090395次级代谢产物进行分离纯化。通过紫外光谱、质谱、核磁共振等波谱学方法对化合物进行结构鉴定。结果与结论分离得到化合物1和化合物2,结构解析确定它们分别与proximicin A和B同质,活性研究表明化合物1对肿瘤细胞株MDA-MB-231(乳腺癌细胞)、SW620(结肠癌细胞)、SMMC7721(肝癌细胞)和CNE-2(鼻咽癌细胞)具有一定的抑制作用,其IC50值分别为23.49、13.48、34.96和16.28μg/mL;化合物2对肿瘤细胞株K562(白血病细胞)具有一定的抑制作用,其IC50值为25.69μg/mL,对MDA-MB-231和CNE-2的抑制增殖作用较弱。

Abtract Objective To study the metabolites of Verrucosispora FIM090395 from marine sediments.Methods The metabolites from the culture filtrate of FIM090395 were purified by macroporous resin HP-20,silica gel,sephadex LH-20 and C18 reversed phase column chromatography.The structure of the compounds were determined by UV spectra,LC-MS,1H and 13C-NMR data analyses.Results and Conclusion Two compounds(1 and 2) were isolated from the culture filtrate,they were identified as proximicin A and B,respect...