{Author}:彭 飞 王传喜 江宏磊 江宁宇 谢 阳 陈路劼 江 红 连云阳

{Year}:2013

{Title}:海洋链孢囊菌FIM09-1157产生的神经氨酸酶抑制剂

{Journal}:《天然产物研究所与开发》

{Volume}:25

{Issue}:2

{Pages}: 193-196

{ISBN/ISSN}: 1001-6880

{Keywords}: Streptosporangium 分离纯化 结构 thiotetromycin

{Abstract}:本文对分离自海洋样品具有神经氨酸酶抑制活性的放线菌FIM09-1157进行初步的分类研究,其形态特征和16S rDNA序列分析表明该菌属于链孢囊菌属(Streptosporangium sp.)。采用硅胶柱色谱、凝胶层析以及高效制备液相对链孢囊菌FIM 09-1157发酵代谢产物进行分离纯化,获得活性化合物T1。理化性质和UV、MS以及1D和2D-NMR波谱分析结果表明化合物T1与链霉菌产生的硫内酯类化合物Thiotetromycin同质。本文首次报道了化合物T1体外神经氨酸酶抑制活性,其IC50值为92μmol/L。

Based on morphological characteristics and 16S rDNA sequence analysis,actinomycete strain FIM09-1157,which was isolated from marine sediment,was classified as Streptosporangium sp.The bioactive metabolite T1 was isolated and purified from the fermentation broth of Streptosporangium FIM09-1157 using silica gel chromatography,gel chromatography and high performance preparative chromatography.Basic physico-chemical properties and UV,MS and 1D,2D-NMR spectral data revealed that compound T1 was ident...